MEG Software and Analysis: Difference between revisions

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==== MEG Data Analysis ====
==== Using the CTF DataEditor with a Singularity Container ====
The current version of the '''CTF DataEditor tool''' runs only under CentOS Linux version 6.9. To support the MEG user community,
[mailto:holroydt@mail.nih.gov Tom Holroyd] has created a [https://singularity.lbl.gov/index.html Singularity] container for CentOS 6.9 and populated the container with the necessary libraries to run the DataEditor tool. This allows MEG users to run the DataEditor tool under the operating system (Windows, Mac OSX, Linux) of their choice.


This section covers all aspects of MEG data analysis. The following pages assume that you have [[afni.nimh.nih.gov|AFNI]] installed and have a reasonably good idea of how to use it.
Singularity is a
[https://en.wikipedia.org/wiki/Operating-system-level_virtualization Operating-system-level virtualization] solution where an operating system
can host another operating system in an isolated container. To run a Singularity container on your operating system, you need to install the Singularity software for your operating system. There are install packages for the major operating systems from the [https://singularity.lbl.gov/index.html Singularity website.]
----
; Singularity container running CentOS 6.7 with the CTF DataEditor tool
: [https://megcore.nih.gov/MEG/ctf-6.1.14-beta.img Singularity container ctf-6.1.14-beta.img download]
: {| width=70% style="border: 1px solid black;"
|<u>Usage</u>: singularity shell --bind /data:/mnt/data ctf-6.1.14-beta.img
|-
| where the ''/data'' file system holds your CTF data set and mounts under /mnt/data inside the container
|-
| (your /home directory and the /tmp file systems are automatically visible inside the container)
|}


* [[CTF Tools|The CTF Tools in a Singularity Container]]
; felix.nih.gov users
: ssh -X login@felix.nih.gov
: copy the ctf-6.1.14-beta.img to your /data directory.


* [[Pyctf|Accessing CTF Datasets from Python]]
: either add '''module load singularity''' to your ~/.bashrc startup file or type this on the command line
: use the following bash script to shell into the singularity container


* [[Time Frequency Analysis|Time Frequency Analysis Tools]]
<syntaxhighlight lang="bash">
#!/bin/bash
# goctf.sh


* [[Head Localization and MRI Coregistration|Head Localization and MRI Co-Registration]]
singularity shell --bind /data/username:/mnt/data ctf-6.1.14-beta.img
killall -s msgd 2>/dev/null
</syntaxhighlight>


* [[Source Localization - SAM|Source Localization - the SAM pipeline]]
The DataEditor communicates with other CTF tools using the '''msgd''' service and the DataEditor will automatically
launch this service if it isn't running. The trouble on felix.nih.gov is this service is not killed when you exit from the singularity container and you will end up with a persistent process on felix. to prevent this from occurring, make sure msgd goes away.


* [[Fun Stuff - How to make a movie| Fun Stuff - How to make a movie ]]
You may want to add this stanza to your ~/.bashrc file on felix.nih.gov.
This sets the command prompt and the command history format for inside the container


* [[MEG analysis on Biowulf| MEG analysis on Biowulf]]
<syntaxhighlight lang="bash">
if [ ! -z "$SINGULARITY_CONTAINER" ]; then
## PS1 is set inside the container
HISTFILE=~/.bash_history
HISTTIMEFORMAT='%Y%m%d/%H:%M:%S '
else
export PS1='[\h \w]$ '
fi
</syntaxhighlight>


* [[Mne bids pipeline | MNE Bids Pipeline and BIDS background]]
===== MEG Data Analysis =====


* [[External MEG Analysis Toolboxes | Other Software packages]]
This section covers all aspects of MEG data analysis. The following pages assume that you have [[afni.nimh.nih.gov|AFNI]] installed and have a reasonably good idea of how to use it.


* Converting Brainsight Localizers or AFNI fiducials [https://github.com/nih-megcore/nih_to_mne to tags or MNE transforms]
* [[Dataset and Task Utilities|Dataset and Task Utilities]]


* Connectivity Analysis [[Connectivity Resources | Resources]]
* [[Time Frequency Analysis|Time Frequency Analysis Tools]]


* ICA Cleaning/Analysis [[ICA cleaning | ICA]]
* [[Head Localization and MRI Coregistration|Head Localization and MRI Co-Registration]]


* Github MEG Code for NIH Labs doing MEG research [[NIH Labs Github Pages | Github Links]]
* [[Source Localization - SAM|Source Localization - the SAM pipeline]]


==== Basic Tutorials for New/Inexperienced Researchers====
* [[Fun Stuff - How to make a movie| Fun Stuff - How to make a movie ]]

Most MEG Core scripts are written in bash, which is the command line interface to Linux. Python is a more powerful programming language, and Tom and Jeff have written some of our scripts in Python, but you don't really need to know how they work, just what they do. The AFNI tutorial below is geared towards fMRI/BOLD analysis. For MEG analysis you can ignore all the BOLD things, we just use it for statistics and display of the final source reconstruction results. If you want to go more in depth, Tom can explain the ones we use. Biowulf can be employed later when you want it all to go faster.

* HPC: Introduction to Linux -- https://hpc.nih.gov/training/handouts/Introduction_to_Linux.pdf
* HPC: Bash Class -- https://hpc.nih.gov/training/bash_class/
* Python Tutorial -- https://docs.python.org/3/tutorial/
* AFNI Introduction -- https://andysbrainbook.readthedocs.io/en/latest/AFNI/AFNI_Short_Course/AFNI_fMRI_Intro.html
* HPC: Introduction to Biowulf -- https://hpc.nih.gov/training/intro_biowulf


==== Stimulus Presentation Software ====

'''PsychoPy: Psychology software in Python'''
[http://www.psychopy.org PsychoPy] is an open-source application that allows you to run a wide range of neuroscience, psychology and psychophysics experiments. It’s a free, powerful alternative to Presentation™ or to e-Prime™, written in Python (a free alternative to Matlab™ ).
*[[Image:pdf.png]] [[Media:PsychoPyManual.pdf| Pyschopy documentation for release 1.90.2]]

'''Presentation: NeuroBehavioral Systems (NBS), Inc.'''
Presentation® is a stimulus delivery and experiment control program for neuroscience written for Microsoft Windows.
* [https://www.neurobs.com/presentation/docs/index_html Presentation online documentation]

'''E-prime 3: Psychology Software Tools'''
E-Prime® 3.0 software for behavioral research. ''Build your own experiments using E-Prime’s easy-to-use graphical interface. Design, collect, and analyze data – all within a few hours!''
* [https://pstnet.com/products/e-prime/ E-Prime 3.0 website]

====Miscellaneous Documentation====


* [[Image:adobe-ps.png]] [[Media:SensLayout-275.ps|SensLayout-275]] — a color picture showing the sensor names and relative locations (ps).
* [[External MEG Analysis Toolboxes | External MEG Analysis Toolboxes]]
* [[Image:pdf.png]] [[Media:SensLayout-275.pdf|SensLayout-275]] - a color picture showing the sensor names and relative locations (pdf).
* [[Image:pdf.png]] [[Media:FileFormats.pdf|File Formats]] - CTF MEG data file format

Latest revision as of 12:20, 25 October 2022

MEG Data Analysis

This section covers all aspects of MEG data analysis. The following pages assume that you have AFNI installed and have a reasonably good idea of how to use it.

  • ICA Cleaning/Analysis ICA

Basic Tutorials for New/Inexperienced Researchers

Most MEG Core scripts are written in bash, which is the command line interface to Linux. Python is a more powerful programming language, and Tom and Jeff have written some of our scripts in Python, but you don't really need to know how they work, just what they do. The AFNI tutorial below is geared towards fMRI/BOLD analysis. For MEG analysis you can ignore all the BOLD things, we just use it for statistics and display of the final source reconstruction results. If you want to go more in depth, Tom can explain the ones we use. Biowulf can be employed later when you want it all to go faster.


Stimulus Presentation Software

PsychoPy: Psychology software in Python PsychoPy is an open-source application that allows you to run a wide range of neuroscience, psychology and psychophysics experiments. It’s a free, powerful alternative to Presentation™ or to e-Prime™, written in Python (a free alternative to Matlab™ ).

Presentation: NeuroBehavioral Systems (NBS), Inc. Presentation® is a stimulus delivery and experiment control program for neuroscience written for Microsoft Windows.

E-prime 3: Psychology Software Tools E-Prime® 3.0 software for behavioral research. Build your own experiments using E-Prime’s easy-to-use graphical interface. Design, collect, and analyze data – all within a few hours!

Miscellaneous Documentation

  • SensLayout-275 — a color picture showing the sensor names and relative locations (ps).
  • SensLayout-275 - a color picture showing the sensor names and relative locations (pdf).
  • File Formats - CTF MEG data file format